Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.

For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.

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Detalles Bibliográficos
Autores principales: Hashimoto, Y, Itho, Y, Fujinaga, Y, Khan, A Q, Sultana, F, Miyake, M, Hirose, K, Yamamoto, H, Ezaki, T
Formato: Artigo
Lenguaje:Inglês
Publicado: 1995
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC228036/
https://ncbi.nlm.nih.gov/pubmed/7751397
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