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Specific detection of Brucella DNA by PCR.

A PCR assay with primers derived from the 16S rRNA sequence of Brucella abortus was developed. Nine different combinations between six primers were tested. One pair of primers, which amplified a 905-bp fragment, was selected. As little as 80 fg of Brucella DNA was detected by this method. DNAs from...

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Bibliografische gegevens
Gepubliceerd in:J Clin Microbiol
Hoofdauteurs: Romero, C, Gamazo, C, Pardo, M, López-Goñi, I
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1995
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC227999/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7538508/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.3.615-617.1995
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