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Unassisted refolding of urea-denatured arginine kinase from shrimp Feneropenaeus chinensis: Evidence for two equilibrium intermediates in the refolding pathway

The refolding process and the equilibrium intermediates of urea-denatured arginine kinase (AK) were investigated by 1-anilino-8-naphthalenesulfonate (ANS) intrinsic fluorescence, far-UV circular dichroism (CD), size-exclusion chromatography (SEC), and enzymatic activity. In dilute denaturant, two eq...

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Detalhes bibliográficos
Main Authors: Pan, Ji-Cheng, Yu, Zhenhang, Su, Xiao-Yang, Sun, Ye-Qing, Rao, Xue-Ming, Zhou, Hai-Meng
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279925/
https://ncbi.nlm.nih.gov/pubmed/15215531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03464804
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