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The Use of Real-Time Reverse Transcriptase PCR for the Quantification of Cytokine Gene Expression

Real-time reverse transcriptase polymerase chain reaction (RT-PCR) is becoming a widely used method to quantify cytokines from cells, tissues, or tissue biopsies. The method allows for the direct detection of PCR product during the exponential phase of the reaction, combining amplification and detec...

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Autors principals: Overbergh, L., Giulietti, A., Valckx, D., Decallonne, B., Bouillon, R., Mathieu, C.
Format: Artigo
Idioma:Inglês
Publicat: The Association of Biomolecular Resource Facilities 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279895/
https://ncbi.nlm.nih.gov/pubmed/12901609
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