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The Use of Real-Time Reverse Transcriptase PCR for the Quantification of Cytokine Gene Expression

Real-time reverse transcriptase polymerase chain reaction (RT-PCR) is becoming a widely used method to quantify cytokines from cells, tissues, or tissue biopsies. The method allows for the direct detection of PCR product during the exponential phase of the reaction, combining amplification and detec...

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Autori principali: Overbergh, L., Giulietti, A., Valckx, D., Decallonne, B., Bouillon, R., Mathieu, C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Association of Biomolecular Resource Facilities 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279895/
https://ncbi.nlm.nih.gov/pubmed/12901609
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