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An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins

The recombinant expression of integral membrane proteins is considered a major challenge, and together with the crystallization step, the major hurdle toward routine structure determination of membrane proteins. Basic methodologies for high-throughput (HTP) expression optimization of soluble protein...

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Detalhes bibliográficos
Main Authors: Eshaghi, Said, Hedrén, Marie, Nasser, Marina Ignatushchenko Abdel, Hammarberg, Tove, Thornell, Anders, Nordlund, Pär
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279288/
https://ncbi.nlm.nih.gov/pubmed/15689514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.041127005
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