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Crystallization of bacteriorhodopsin from bicelle formulations at room temperature

We showed previously that high-quality crystals of bacteriorhodopsin (bR) from Halobacterium salinarum can be obtained from bicelle-forming DMPC/CHAPSO mixtures at 37°C. As many membrane proteins are not sufficiently stable for crystallization at this high temperature, we tested whether the bicelle...

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Autors principals: Faham, Salem, Boulting, Gabriella L., Massey, Elizabeth A., Yohannan, Sarah, Yang, Dawn, Bowie, James U.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279272/
https://ncbi.nlm.nih.gov/pubmed/15689517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.041167605
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