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3’-(32)P Labeling tRNA with Nucleotidyltransferase for Assaying Aminoacylation and Peptide Bond Formation
The analysis of reactions involving amino acids esterified to tRNAs traditionally uses radiolabeled amino acids. We describe here an alternative assay involving [3’-(32)P] labeled tRNA followed by nuclease digestion and TLC analysis that permits aminoacylation to be monitored in an efficient, quanti...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2275914/ https://ncbi.nlm.nih.gov/pubmed/18241789 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2007.08.001 |
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