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Sequence-function relationships provide new insight into the cleavage site selectivity of the 8–17 RNA-cleaving deoxyribozyme

Many sequence variations of the 8–17 RNA-cleaving deoxyribozyme have been isolated through in vitro selection. In an effort to understand how these sequence variations affect cleavage site selectivity, we systematically mutated the catalytic core of 8–17 and measured the cleavage activity of each mu...

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Autors principals: Schlosser, Kenny, Gu, Jimmy, Sule, Lauren, Li, Yingfu
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2008
Matèries:
RNA
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2275145/
https://ncbi.nlm.nih.gov/pubmed/18203744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm1175
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