Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression

Production of structure-grade mammalian membrane proteins in substantial quantities has been hindered by a lack of methods for effectively profiling multiple construct expression in higher eukaryotic systems such as insect or mammalian cells. To address this problem, a specialized small-scale eukary...

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Autori principali: Hanson, Michael A., Brooun, Alexei, Baker, Kent A., Jaakola, Veli-Pekka, Roth, Chris, Chien, Ellen, Alexandrov, Alexander, Velasquez, Jeffrey, Davis, Leila, Griffith, Mark, Moy, Kin, Ganser-Pornillos, Barbie, Kuhn, Peter, Ellis, Sam, Yeager, Mark, Stevens, Raymond C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2007
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2274776/
https://ncbi.nlm.nih.gov/pubmed/17723307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.06.003
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