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Identifying the site of spin-trapping in proteins by a combination of liquid chromatography, ELISA and off-line tandem mass spectrometry
An off-line mass spectrometry method that combines immuno-spin trapping and chromatographic procedures has been developed for selective detection of the nitrone spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) covalently attached to proteins, an attachment which occurs only subsequent to DMPO trapp...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2268891/ https://ncbi.nlm.nih.gov/pubmed/18160050 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2007.11.015 |
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