Identifying the site of spin-trapping in proteins by a combination of liquid chromatography, ELISA and off-line tandem mass spectrometry

An off-line mass spectrometry method that combines immuno-spin trapping and chromatographic procedures has been developed for selective detection of the nitrone spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) covalently attached to proteins, an attachment which occurs only subsequent to DMPO trapp...

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Bibliographic Details
Main Authors: Lardinois, Olivier M., Detweiler, Charles D., Tomer, Kenneth B., Mason, Ronald P., Deterding, Leesa J.
Format: Artigo
Language:Inglês
Published: 2007
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2268891/
https://ncbi.nlm.nih.gov/pubmed/18160050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2007.11.015
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