A nanostructure-initiator mass spectrometry-based enzyme activity assay

We describe a Nanostructure-Initiator Mass Spectrometry (NIMS) enzymatic (Nimzyme) assay in which enzyme substrates are immobilized on the mass spectrometry surface by using fluorous-phase interactions. This “soft” immobilization allows efficient desorption/ionization while also enabling the use of...

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Bibliographic Details
Main Authors: Northen, Trent R., Lee, Jinq-Chyi, Hoang, Linh, Raymond, Jason, Hwang, Der-Ren, Yannone, Steven M., Wong, Chi-Huey, Siuzdak, Gary
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2008
Subjects:
Physical Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2268803/
https://ncbi.nlm.nih.gov/pubmed/18319341
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0712332105
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