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A nanostructure-initiator mass spectrometry-based enzyme activity assay
We describe a Nanostructure-Initiator Mass Spectrometry (NIMS) enzymatic (Nimzyme) assay in which enzyme substrates are immobilized on the mass spectrometry surface by using fluorous-phase interactions. This “soft” immobilization allows efficient desorption/ionization while also enabling the use of...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2268803/ https://ncbi.nlm.nih.gov/pubmed/18319341 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0712332105 |
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