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A promoter-hijack strategy for conditional shutdown of multiply spliced essential cell cycle genes
We describe a method for the isolation of conditional knockouts of essential multiply spliced genes in which the entire body of the gene downstream of the ATG start codon is left untouched but can be switched off rapidly and completely by adding tetracycline to the culture medium. The approach cente...
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| 主要な著者: | , , , , , , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2008
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2268158/ https://ncbi.nlm.nih.gov/pubmed/18263736 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0712083105 |
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