A promoter-hijack strategy for conditional shutdown of multiply spliced essential cell cycle genes

We describe a method for the isolation of conditional knockouts of essential multiply spliced genes in which the entire body of the gene downstream of the ATG start codon is left untouched but can be switched off rapidly and completely by adding tetracycline to the culture medium. The approach cente...

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Detalhes bibliográficos
Main Authors: Samejima, Kumiko, Ogawa, Hiromi, Cooke, Carol A., Hudson, Damien, MacIsaac, Fiona, Ribeiro, Susana A., Vagnarelli, Paola, Cardinale, Stefano, Kerr, Alastair, Lai, Fan, Ruchaud, Sandrine, Yue, Zuojun, Earnshaw, William C.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2008
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2268158/
https://ncbi.nlm.nih.gov/pubmed/18263736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0712083105
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