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Recombinant proteinase 3 (Wegener's antigen) expressed in Pichia pastoris is functionally active and is recognized by patient sera

The open reading frame of human proteinase 3 (PR3) without the prepro-peptide was cloned and expressed in Escherichia coli (rcPR3) and in Pichia pastoris (rpPR3). The 6-histidine tagged rpPR3 was efficiently secreted into culture supernatant from which it could be purified by immobilized metal chela...

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Detaylı Bibliyografya
Asıl Yazarlar: HARMSEN, M C, HEERINGA, P, VAN DER GELD, Y M, HUITEMA, M G, KLIMP, A, TIRAN, A, KALLENBERG, C G M
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Blackwell Science Inc 1997
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2265502/
https://ncbi.nlm.nih.gov/pubmed/9367410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2249.1997.tb08325.x
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