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Cooperative α-helix unfolding in a protein–DNA complex from hydrogen–deuterium exchange

We present experimental evidence for a cooperative unfolding transition of an α-helix in the lac repressor headpiece bound to a symmetric variant of the lac operator, as inferred from hydrogen–deuterium (H–D) exchange experiments monitored by NMR spectroscopy. In the EX1 limit, observed exchange rat...

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Detalhes bibliográficos
Main Authors: Salinas, Roberto K., Diercks, Tammo, Kaptein, Robert, Boelens, Rolf
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2265102/
https://ncbi.nlm.nih.gov/pubmed/16751603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051938006
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