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Three-state protein folding: Experimental determination of free-energy profile

When considering protein folding with a transient intermediate, a difficulty arises as to determination of the rates of separate transitions. Here we overcome this problem, using the kinetic studies of the unfolding/refolding reactions of the three-state protein apomyoglobin as a model. Amplitudes o...

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Detalhes bibliográficos
Main Authors: Baryshnikova, Ekaterina N., Melnik, Bogdan S., Finkelstein, Alexei V., Semisotnov, Gennady V., Bychkova, Valentina E.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2253297/
https://ncbi.nlm.nih.gov/pubmed/16155199
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051402705
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