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Engineering covalent oligomers of the mechanosensitive channel of large conductance from Escherichia coli with native conductance and gating characteristics
To obtain a gene construct for making single substitutions per channel and to determine the quaternary structure of the mechanosensitive channel MscL from Escherichia coli, covalent oligomers (monomer to hexamer) were engineered by gene fusion; up to six copies of the mscL gene were fused in tandem....
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2005
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2253253/ https://ncbi.nlm.nih.gov/pubmed/16322576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051679005 |
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