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Crystal structure of the E230Q mutant of cAMP-dependent protein kinase reveals an unexpected apoenzyme conformation and an extended N-terminal A helix

Glu230, one of the acidic residues that cluster around the active site of the catalytic subunit of cAMP-dependent protein kinase, plays an important role in substrate recognition. Specifically, its side chain forms a direct salt-bridge interaction with the substrate’s P-2 Arg. Previous studies showe...

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Detalhes bibliográficos
Main Authors: Wu, Jian, Yang, Jie, Kannan, Natarajan, Madhusudan, Xuong, Nguyen-Huu, Ten Eyck, Lynn F., Taylor, Susan S.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2253214/
https://ncbi.nlm.nih.gov/pubmed/16253959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051715205
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