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Mutations Affecting DNA-Binding Activity of the MexR Repressor of mexR-mexA-mexB-oprM Operon Expression

We have isolated 25 MexR mutants that retained their dimerizing ability but were unable to bind mexOP DNA. Surprisingly, 20 mutations were located in the hydrophobic core region at α4, W1, α2, α3, and β2, and only 3 were in positively charged residues. These results verified that DNA binding is medi...

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Detalhes bibliográficos
Main Authors: Saito, Kohjiro, Akama, Hiroyuki, Yoshihara, Eisaku, Nakae, Taiji
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC225045/
https://ncbi.nlm.nih.gov/pubmed/14526032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.20.6195-6198.2003
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