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A ``loop entropy reduction'' phage-display selection for folded amino acid sequences

As a step toward selecting folded proteins from libraries of randomized sequences, we have designed a `loop entropy reduction'-based phage-display method. The basic premise is that insertion of a long disordered sequence into a loop of a host protein will substantially destabilize the host beca...

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Bibliografische gegevens
Hoofdauteurs: Minard, Philippe, Scalley-Kim, Michelle, Watters, Alex, Baker, David
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Cold Spring Harbor Laboratory Press 2001
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2249851/
https://ncbi.nlm.nih.gov/pubmed/11266601
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