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Site-specific labeling of proteins for single-molecule FRET by combining chemical and enzymatic modification

An often limiting factor for studying protein folding by single-molecule fluorescence resonance energy transfer (FRET) is the ability to site-specifically introduce a photostable organic FRET donor (D) and a complementary acceptor (A) into a polypeptide chain. Using alternating-laser excitation and...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Jäger, Marcus, Nir, Eyal, Weiss, Shimon
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Cold Spring Harbor Laboratory Press 2006
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2249784/
https://ncbi.nlm.nih.gov/pubmed/16452617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051851506
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