Simultaneous determination of helical unwinding angles and intrinsic association constants in ligand–DNA complexes: The interaction between DNA and calichearubicin B

We present a helical unwinding assay for reversibly binding DNA ligands that uses closed circular DNA, topoisomerase I (Topo I), and two-dimensional agarose gel electrophoresis. Serially diluted Topo I relaxation reactions at constant DNA/ligand ratio are performed, and the resulting apparent unwind...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Zeman, Steven M., Depew, Kristopher M., Danishefsky, Samuel J., Crothers, Donald M.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: The National Academy of Sciences 1998
Gaiak:
Biological Sciences
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC22488/
https://ncbi.nlm.nih.gov/pubmed/9539736
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