Simultaneous determination of helical unwinding angles and intrinsic association constants in ligand–DNA complexes: The interaction between DNA and calichearubicin B

We present a helical unwinding assay for reversibly binding DNA ligands that uses closed circular DNA, topoisomerase I (Topo I), and two-dimensional agarose gel electrophoresis. Serially diluted Topo I relaxation reactions at constant DNA/ligand ratio are performed, and the resulting apparent unwind...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Zeman, Steven M., Depew, Kristopher M., Danishefsky, Samuel J., Crothers, Donald M.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1998
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22488/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9539736/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.8.4327
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