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Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD
The flavoprotein cholesterol oxidase (CO) from Brevibacterium sterolicum is a monomeric flavoenzyme containing one molecule of FAD cofactor covalently linked to His69. The elimination of the covalent link following the His69Ala substitution was demonstrated to result in a significant decrease in act...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2248306/ https://ncbi.nlm.nih.gov/pubmed/18218720 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073137708 |
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