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Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris

Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cle...

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Hlavní autoři: Zhou, Jin, Chu, Ju, Wang, Yong-Hong, Wang, Hui, Zhuang, Ying-Ping, Zhang, Si-Liang
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer Netherlands 2007
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2248218/
https://ncbi.nlm.nih.gov/pubmed/18060365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-007-9610-4
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