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Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos

BACKGROUND: The formation of two distinctive cell lineages in preimplantation mouse embryos is characterized by differential gene expression. The cells of the inner cell mass are pluripotent and express high levels of Oct4 mRNA, which is down-regulated in the surrounding trophectoderm. In contrast,...

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Detalhes bibliográficos
Main Authors: Hartshorn, Cristina, Eckert, Judith J, Hartung, Odelya, Wangh, Lawrence J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2246118/
https://ncbi.nlm.nih.gov/pubmed/18067662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-87
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