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A Method to Determine (18)O Kinetic Isotope Effects in the Hydrolysis of Nucleotide Triphosphates
A method to determine (18)O kinetic isotope effects (KIE) in the hydrolysis of GTP is described that is generally applicable to reactions involving other nucleotide triphosphates. Internal competition, wherein the substrate of the reaction is a mixture of (18)O-labeled and unlabeled nucleotides, is...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2245892/ https://ncbi.nlm.nih.gov/pubmed/17963711 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.09.013 |
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