Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540

A gene encoding a putrescine oxidase (PuO(Rh), EC 1.4.3.10) was identified from the genome of Rhodococcus erythropolis NCIMB 11540. The gene was cloned in the pBAD vector and overexpressed at high levels in Escherichia coli. The purified enzyme was shown to be a soluble dimeric flavoprotein consisti...

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Autors principals: van Hellemond, Erik W., van Dijk, Marianne, Heuts, Dominic P. H. M., Janssen, Dick B., Fraaije, Marco W.
Format: Artigo
Idioma:Inglês
Publicat: Springer Berlin Heidelberg 2008
Matèries:
Biotechnologically Relevant Enzymes and Proteins
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2243256/
https://ncbi.nlm.nih.gov/pubmed/18183391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-007-1310-4
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