Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540

A gene encoding a putrescine oxidase (PuO(Rh), EC 1.4.3.10) was identified from the genome of Rhodococcus erythropolis NCIMB 11540. The gene was cloned in the pBAD vector and overexpressed at high levels in Escherichia coli. The purified enzyme was shown to be a soluble dimeric flavoprotein consisti...

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Main Authors: van Hellemond, Erik W., van Dijk, Marianne, Heuts, Dominic P. H. M., Janssen, Dick B., Fraaije, Marco W.
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2008
Fag:
Biotechnologically Relevant Enzymes and Proteins
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2243256/
https://ncbi.nlm.nih.gov/pubmed/18183391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-007-1310-4
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