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Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon

Crystallization of and preliminary crystallographic studies on an active-site mutant of pro-Tk-subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis were performed. The crystal was grown at 277 K by the sitting-drop vapour-diffusion method. Native X-ray diffraction data were coll...

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Detalhes bibliográficos
Main Authors: Tanaka, Shun-ichi, Saito, Kenji, Chon, Hyongi, Matsumura, Hiroyoshi, Koga, Yuichi, Takano, Kazufumi, Kanaya, Shigenori
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242867/
https://ncbi.nlm.nih.gov/pubmed/16946475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106030454
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