Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis

Peptidyl-tRNA hydrolase catalyses the cleavage of the ester link between the peptide and the tRNA in peptidyl-tRNAs that, for various reasons, have dropped off the translating ribosomes. This enzyme from Mycobacterium tuberculosis has been crystallized in three related but distinct forms: P2(1)2(1)2...

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Bibliographic Details
Main Authors: Selvaraj, M., Singh, N. S., Roy, Siddhartha, Sangeetha, R., Varshney, Umesh, Vijayan, M.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2006
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242860/
https://ncbi.nlm.nih.gov/pubmed/16946478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106031125
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