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Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor

Uracil N-glycosylase is an enzyme which initiates the pathway of uracil-excision repair of DNA. The enzyme from Mycobacterium tuberculosis was co-expressed with a proteinaceous inhibitor from Bacillus subtilis phage and was crystallized in monoclinic space group C2, with unit-cell parameters a = 201...

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Detalhes bibliográficos
Main Authors: Singh, Prem, Talawar, Ramappa K., Krishna, P. D. V., Varshney, Umesh, Vijayan, M.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225355/
https://ncbi.nlm.nih.gov/pubmed/17142904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106045805
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