Recombination-Mediated Genetic Engineering of a Bacterial Artificial Chromosome Clone of Modified Vaccinia virus Ankara (MVA)

The production, manipulation and rescue of a bacterial artificial chromosome clone of Vaccinia virus (VAC-BAC) in order to expedite construction of expression vectors and mutagenesis of the genome has been described (Domi & Moss, 2002, PNAS 99 12415–20). The genomic BAC clone was ‘rescued’ back...

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Detalhes bibliográficos
Main Authors: Cottingham, Matthew G., Andersen, Rikke F., Spencer, Alexandra J., Saurya, Saroj, Furze, Julie, Hill, Adrian V. S., Gilbert, Sarah C.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2008
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242847/
https://ncbi.nlm.nih.gov/pubmed/18286194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0001638
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