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Native state energetics of the Src SH2 domain: Evidence for a partially structured state in the denatured ensemble
We have defined the free-energy profile of the Src SH2 domain using a variety of biophysical techniques. Equilibrium and kinetic experiments monitored by tryptophan fluorescence show that Src SH2 is quite stable and folds rapidly by a two-state mechanism, without populating any intermediates. Native...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2242571/ https://ncbi.nlm.nih.gov/pubmed/16751610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062136006 |
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