Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen

We have optimized the expression level of 20 mammalian G protein-coupled receptors (GPCRs) in the methylotrophic yeast Pichia pastoris. We found that altering expression parameters, including growth temperature, and supplementation of the culture medium with specific GPCR ligands, histidine, and DMS...

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Detalhes bibliográficos
Main Authors: André, Nicolas, Cherouati, Nadia, Prual, Cécile, Steffan, Tania, Zeder-Lutz, Gabrielle, Magnin, Thierry, Pattus, Franc, Michel, Hartmut, Wagner, Renaud, Reinhart, Christoph
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2006
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242496/
https://ncbi.nlm.nih.gov/pubmed/16597836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062098206
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