A Single Mutation in the Activation Site of Bovine Trypsinogen Enhances Its Accumulation in the Fermentation Broth of the Yeast Pichia pastoris

We produced bovine trypsinogen in the yeast Pichia pastoris. Little or no trypsinogen was detected when the gene with its native leader sequence was expressed under the control of the strong aox1 promoter, suggesting that expression of the wild-type bovine trypsinogen was toxic to the cells. We alte...

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Detalhes bibliográficos
Main Authors: Hanquier, José, Sorlet, Yannick, Desplancq, Dominique, Baroche, Laurence, Ebtinger, Marc, Lefèvre, Jean-François, Pattus, Franc, Hershberger, Charles L., Vertès, Alain A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC143679/
https://ncbi.nlm.nih.gov/pubmed/12571036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.1108-1113.2003
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