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Efficient siRNA selection using hybridization thermodynamics

Small interfering RNA (siRNA) are widely used to infer gene function. Here, insights in the equilibrium of siRNA-target hybridization are used for selection of efficient siRNA. The accessibilities of siRNA and target mRNA for hybridization, as measured by folding free energy change, are shown to be...

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Autors principals: Lu, Zhi John, Mathews, David H.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2241856/
https://ncbi.nlm.nih.gov/pubmed/18073195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm920
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