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Mechanical and chemical unfolding of a single protein: A comparison

Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or a true measurement of their unfolding? By engineering a protein made of tandem repeats of identical Ig modules, we were able to get explicit AFM data on the unfolding rate of a single protei...

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Detalhes bibliográficos
Main Authors: Carrion-Vazquez, Mariano, Oberhauser, Andres F., Fowler, Susan B., Marszalek, Piotr E., Broedel, Sheldon E., Clarke, Jane, Fernandez, Julio M.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC22356/
https://ncbi.nlm.nih.gov/pubmed/10097099
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