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Digitonin-permeabilized colonic cell layers. Demonstration of calcium- activated basolateral K+ and Cl- conductances
Sheets of isolated turtle colon were exposed to digitonin on the mucosal side to chemically remove the apical membrane as a permeability barrier. Increases in the mucosal uptake of 86Rb, [3H]mannitol, and 45Ca-EGTA, and the appearance of the cytosolic marker enzyme lactate dehydrogenase in the mucos...
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| フォーマット: | Artigo |
|---|---|
| 言語: | Inglês |
| 出版事項: |
The Rockefeller University Press
1988
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2228905/ https://ncbi.nlm.nih.gov/pubmed/2465372 |
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