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Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs

MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect...

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Autors principals: Varkonyi-Gasic, Erika, Wu, Rongmei, Wood, Marion, Walton, Eric F, Hellens, Roger P
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2007
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225395/
https://ncbi.nlm.nih.gov/pubmed/17931426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-3-12
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