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Expression, purification, crystallization and preliminary X-ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae

There are three thioredoxin isoforms in the yeast Saccharomyces cerevisiae: two cytosolic/nuclear thioredoxins, Trx1 and Trx2, and one mitochondrial thioredoxin, Trx3. In the present work, S. cerevisiae Trx3 overexpressed in Escherichia coli was purified and crystallized. The Trx3 crystals were obta...

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Autori principali: Bao, Rui, Chen, Yu-xing, Zhang, Yaru, Zhou, Cong-Zhao
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225218/
https://ncbi.nlm.nih.gov/pubmed/17077505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106041467
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