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Expression, purification, crystallization and preliminary X-ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae
There are three thioredoxin isoforms in the yeast Saccharomyces cerevisiae: two cytosolic/nuclear thioredoxins, Trx1 and Trx2, and one mitochondrial thioredoxin, Trx3. In the present work, S. cerevisiae Trx3 overexpressed in Escherichia coli was purified and crystallized. The Trx3 crystals were obta...
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Autori principali: | , , , |
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Natura: | Artigo |
Lingua: | Inglês |
Pubblicazione: |
International Union of Crystallography
2006
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Soggetti: | |
Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2225218/ https://ncbi.nlm.nih.gov/pubmed/17077505 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106041467 |
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