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Expression, purification and crystallization of the Atg5–Atg16 complex essential for autophagy

Atg5 is a novel 34 kDa protein that is covalently modified by Atg12, a ubiquitin-like modifier, and forms a complex with Atg16. The Atg12–Atg5–Atg16 complex localizes to autophagosome precursors and plays an essential role in autophagosome formation. Saccharomyces cerevisiae Atg5 in complex with the...

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Autors principals: Matsushita, Minako, Suzuki, Nobuo N., Fujioka, Yuko, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225175/
https://ncbi.nlm.nih.gov/pubmed/17012802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106036232
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