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Studying interactions of four proteins in the yeast two-hybrid system: Structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein

The yeast two-hybrid system is a powerful technique that detects interactions between two proteins and has been useful in identifying new binding partners. However, the system fails to detect protein–protein interactions that require the presence of additional components of a multisubunit complex. H...

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Bibliografiske detaljer
Main Authors: Pause, Arnim, Peterson, Brett, Schaffar, Gregor, Stearman, Robert, Klausner, Richard D.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC22243/
https://ncbi.nlm.nih.gov/pubmed/10449727
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