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Purification, crystallization and preliminary X-ray characterization of the human GTP fucose pyrophosphorylase

The human nucleotide-sugar metabolizing enzyme GTP fucose pyrophosphorylase (GFPP) has been purified to homogeneity by an affinity chromatographic procedure that utilizes a novel nucleoside analog. This new purification regime results in a protein preparation that produces significantly better cryst...

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Detalhes bibliográficos
Main Authors: Quirk, Stephen, Seley-Radtke, Katherine L.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2222559/
https://ncbi.nlm.nih.gov/pubmed/16582493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106008529
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