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Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.

A clone bearing the structural gene sacB, coding for the exoenzyme levansucrase, was isolated from a library of Bacillus subtilis DNA that was cloned in phage lambda charon 4A on the basis of the transforming activity of the chimeric DNA. This lambda clone also was found to contain the sacR and smo...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Gay, P, Le Coq, D, Steinmetz, M, Ferrari, E, Hoch, J A
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1983
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221793/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6402497/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.153.3.1424-1431.1983
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