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Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome.
The Escherichia coli mtl operon, which contains the cis-dominant regulatory region mtlC, the mannitol-specific enzyme II structural gene mtlA, and the D-mannitol-1-phosphate dehydrogenase structural gene mtlD, was cloned into plasmid pBR322. A 2-kilobase pair fragment of the mtl operon DNA containin...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1983
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC221685/ https://ncbi.nlm.nih.gov/pubmed/6401703 |
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