Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.
A broad-host-range cloning vector, pUI81, was constructed in vitro from plasmids RSF1010 and pSL25 (a pBR322 derivative) and used to assay for transformation in Rhodopseudomonas sphaeroides. Washing cells with 500 mM Tris was an effective means of inducing competence for DNA uptake. Transformation f...
Uloženo v:
| Vydáno v: | J Bacteriol |
|---|---|
| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1982
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6981642/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.152.1.89-97.1982 |
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