Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.

A broad-host-range cloning vector, pUI81, was constructed in vitro from plasmids RSF1010 and pSL25 (a pBR322 derivative) and used to assay for transformation in Rhodopseudomonas sphaeroides. Washing cells with 500 mM Tris was an effective means of inducing competence for DNA uptake. Transformation f...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Fornari, C S, Kaplan, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1982
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6981642/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.152.1.89-97.1982
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