Assessment of Caspase Activities in Intact Apoptotic Thymocytes Using Cell-Permeable Fluorogenic Caspase Substrates

To detect caspase activities in intact apoptotic cells at the single cell level, cell-permeable fluorogenic caspase substrates were synthesized incorporating the optimal peptide recognition motifs for caspases 1, 3/7, 6, 8, and 9. Caspase activities were then assessed at various times after in vitro...

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Autors principals: Komoriya, Akira, Packard, Beverly Z., Brown, Martin J., Wu, Ming-Lei, Henkart, Pierre A.
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 2000
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Original Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2213522/
https://ncbi.nlm.nih.gov/pubmed/10839799
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